Effect of Notch1 on biological behavior of bladder cancer cells
10.3760/cma.j.issn.1000-6702.2009.05.013
- VernacularTitle:靶向沉默Notch1基因对人膀胱癌细胞生物学行为的影响
- Author:
Xing AI
;
Baojun WANG
;
Hua XU
;
Taoping SHI
;
Zhenghua JUN
;
Shuanglin LIU
;
Zhun WU
;
Xin MA
;
Hongzhao LI
;
Xu ZHANG
- Publication Type:Journal Article
- Keywords:
Gene silencing;
Receptor,Notch1;
Bladder neoplasms;
Cell proliferation
- From:
Chinese Journal of Urology
2009;30(5):328-331
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the in vitro effects of bladder cancer cell proliferation after silencing Notch1 gene. Methods The siRNA eukaryotic expression vector of Notch1 (psiRNA1)was constructed and transfected into bladder cancer cell lines T24 and BIU-87. Methabensthiazuron (MTT) and flow cytometry (FCM) assays were used to detect bladder cancer cells line growth, cell cycle and apoptosis after the transfection. RT-PCR and Western blotting were used to determine the expression changes of Notch1 in these cell lines. Results After transfection for 72 h, the rate of G0/G1 phase cells inceased from (23.89±1.32) % to (80.13±2.69)% in T24 cell line, and increased from (24.63±1.68)% to (69.44±2.41)% in BIU-87 cell line (both P<0.05). In addition, apop-totic cell index in T24 and BIU-87 cell lines increased from (1.28±0.14)% to (13.75±1.23)%, from (1.01±0.27)% to (8.72±1.01)%, respectively(both P<0.05). The growth of T24 and BIU-87 cell lines was obviously inhibited 24 h after the transfection, and the inhibitory effects lasted until 96 h after the transfection. Notch1 mRNA and protein significantly downregulated after transfection compared to the control(P<0.05). Conclusions Silencing Notch1 expression can inhibit the prolif-eration of bladder cancer cell lines. Notch1 gene might act as a tumor gene in bladder cancer.
