Effects of rapamycin and paclitaxel on different human prostate cancer cells and xenografts
10.3760/cma.j.issn.1673-4203.2009.07.010
- VernacularTitle:雷帕霉素和紫杉醇对激素非依赖性前列腺癌细胞株影响的体外实验研究
- Author:
Qingjun LIU
;
Xiuhong XU
;
Ye TIAN
;
Wencheng LV
;
Qiang SHAO
;
Yuhai ZHANG
- Publication Type:Journal Article
- Keywords:
rapamycin;
paclitaxel;
LNCaP-C4;
LNCaP-C4-2;
PC-3
- From:
International Journal of Surgery
2009;36(7):456-459
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the anti-proliferation effects of rapamycin and paclitaxel of different hu-man prostate cancer cells in vitro. Methods The methods of MTr and flow cytometry were respectively ap-plied to observe the effect of rapamycin, paclitaxel and rapamycin+paclitaxel on proliferation and apoptosis of different prostate cancer cell lines (LNCaP-C4, LNCaP-C4-2, PC-3). Results When the concentration of rapamycin was 0.01 μmol/L, the impressive effect showed a remarkable difference in contrast to the con-trol. While in group LNCaP-C4-2 and PC-3, the goal concentration of rapamycin was 0.001μmol/L. When the concentration of paclitaxel was 0. 2 ng/mL, the impressive effect showed a remarkable difference in con-trast to the control. In group rapamycin (10 nmol/L) and in group paclitaxel (1 ng/mL) there were signifi-cant differences in growth inhibition, compared with control. While in group rapamycin(5 nmoL/L)+pacli-taxel(0.5ng/mL) there was significant difference in growth inhibition, compared with rapamycin (10 nmol/L) and paclitaxel (1 ng/mL) respectively. After cultured with rapamycin or paclitaxel alone, more tumor cells induced apoptosis than control. While after cultured with rapamycin and paclitaxel simultaneously, more tumor cells induced apoptosis than with rapamycin or paclitaxel alone. Conclusions Both rapamycin and paclitaxel had a good impressive effect on the three prostate cell lines (LNCaP-CA, LNCaP-C4-2, PC-3) with dose-dependent manner. After cultured with rapamycin and paclitaxel simultaneously, more tumor cells were induced apoptosis than with rapamycin or paclitaxel alone.
