In vitro study on immunoreaction of dendritic cell vaccine in renal cell carcinoma
10.3760/cma.j.issn.1000-6702.2009.08.004
- VernacularTitle:肾癌树突状细胞疫苗的体外活性研究
- Author:
Changhui FAN
;
Baoping QIAO
;
Jianguo WEN
;
Yuanming QI
;
Qiao RUAN
- Publication Type:Journal Article
- Keywords:
Kidney noeplasms;
Carcinoma;
Vaccines;
Dendritic cells
- From:
Chinese Journal of Urology
2009;30(8):515-517
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the antitumor effect of specific cytotoxic T lymphocytes induced by renal cell carcinoma multiple antigen peptide dendritic cell (DC) vaccine in vitro. Methods Dendritic cells were induced by hGM-CSF,hIL-4 from blood.Peptide of renal cell carcinoma cell line (RCC786-0)was got by citrate-phosphate buffer elution.Peripheral blood mononuclear cell was cultured.Multiple antigen peptide DC cell vaccine was obtained by acid-eluted peptide pulsed DC.The tumor antigen specific CTL was generated from activated T cell by vaccine.Killing activity of the tumor antigen specific CTL was activated by vaccine. Results Antigen peptide DC cell vaccine could show a strong cytotoxic activity of CTLs(31.93±5.05%),which was much higher than control groups(5.88±2.26%,8.03±6.70%,9.70±2.09%,9.35±3.58%). Conclusion Renal cell carcinoma antigen peptide DC vaccine could show a high antitumor effect in vitro.
