Effects of local hyperthermia on phosphatidylinositol 3-kinase gene expression in Langerhans cells from human papillomavirus(HPV)-infected skin
10.3760/cma.j.issn.0412-4030.2009.11.009
- VernacularTitle:温热对HPV感染皮肤朗格汉斯细胞磷酸肌醇3-激酶活性的影响
- Author:
Xiaodong LI
;
Xinghua GAO
;
Yuxiao HONG
;
Lili ZHU
;
Yakun WANG
;
Yubo LIU
;
Wei HUO
;
Xiaoqin WANG
;
Li ZHANG
;
Hongduo CHEN
- Publication Type:Journal Article
- Keywords:
Langerhans cells;
1-Phosphatidylinositul 3-kinase;
Hyperthermia;
induced
- From:Chinese Journal of Dermatology
2009;42(11):756-759
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of local hyperthermia on the mRNA expression of phosphatidylinositol 3-kinase(P13-K)in Langerhans cells(LCs)transmigrating from HPV-infected skin.Methods Tissue samples were collected from 5 female patients with condyloma acuminatum(CA)and 5 normal human controls.then equally divided into three parts to receive local hyoerthermia treatment at 37℃,42 ℃ and 45℃,respectively,for 30 minutes.After another 12-hour incubation in RPMI 1640 culture medium,transmigrating cells were collected and LCs were purified.Real-time quantitative reverse transcription-PCR was performed to detect the mRNA expressions of P13-K p85a and p110α in purified CD1a~+ LCs from these tissue samples.Results In CD1a~+ LCs transmigrating from normal skin and lesions of CA.the expressions of P13-K p85a and P110α mRNA decreased with the increase of temperature.Atier local hyperthermia treatment of normal skin at 37℃,42℃,45℃,the relative mRNA expression level was 1.00±0.00,0.78±0.13,0.54±0.17,respectively.for P13-K p85α in transmigrating LCs,1.00±0.00,0.80±0.11,0.61±0.12,respectively.for P13-K P110α;there was a significant difierence among the three temperatures in bOth parameters(both P＜0.01).In the case of lesions of CA.the relative mRNA expression level of P13-K p85αand p110α was 1.00±0.00 and 1.00±0.00 under treatment at 37℃,0.20±0.11 and 0.49±0.21 at 42℃.0.1±0.08 and 0.09±0.03 at 45℃.respectively;significant difierence was also noted among the three treatment temperatures(both P＜0.01).The decrease in P13-K P110α mRNA expressions under hyperthermia treatment at 42 ℃ and 45℃ compared with those at 37℃was greater in LCs from CA lesions than that from normal skin.Conclusions Local hyoerthermia could remarkably inhibit the expression of P13-K genes.which may enhance immunity and favor elimination of HPV.
