Expression of M3 subtype of muscarinic receptors in normal,BPH and prostate cncer tissue

VernacularTitle:前列腺癌和良性前列腺增生组织中M3受体表达及意义
Author: Mingzhen YUAN ; Shengtian ZHAO ; Yan MENG ; Hongwei WANG ; Chunxiao XU
Publication Type:Journal Article
Keywords: Prostatic neoplasms; Prostatic hyperplasia; Receptor,muscarinic M3; MVD
From: Chinese Journal of Urology 2009;30(8):546-549
CountryChina
Language:Chinese
Abstract: Objective To study the relationship between M3 receptor and prostastic tumor by analyzing the expressions of M3 and vascular endothelial growth factor(VEGF)in adult human normal and neoplastic prostatic gland tissue. Methods The specimens included 36 normal prostates(fresh),36 benign prostatic hyperplasia(BPH)tissue(fresh),and 36 cancer tissue(8 fresh).RT-PCR was used to detect M3 receptor,VEGPs genetic expression.At protein level,VEGF,Ms receptor,CD34 were detected by western-blot and immunohistochemical method. Results VEGF and M3 receptor's genetic expressions were higher in prostate cancer tissue(O.8354±0.1897,0.7824±0.2047)than in BPH tissue(0.6735±0.1603,0.6021±0.1637),while the expressions of these genes were lowest in normal prostate tissue(0.5425±0.1629,0.3436±0.1581)(P<0.001).There was a positive correlation between M3 and VEGFs gene expression(r=0.4999,P