Dynamic changes in glutamate and γ-aminobutyric acid release in the rostral ventromedial medulla in a rat model of incisional pain
10.3760/cma.j.issn.0254-1416.2010.01.014
- VernacularTitle:切口痛大鼠脑干嘴端腹内侧髓质谷氨酸和γ-氨基丁酸释放的动态变化
- Author:
Yun WANG
;
Chunsheng FENG
;
Zhiguo WU
;
Xiaobo MU
;
Anshi WU
;
Yue YUE
- Publication Type:Journal Article
- Keywords:
Amino acids;
Pain;
Medulla oblongata
- From:
Chinese Journal of Anesthesiology
2010;30(1):47-49
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the time-dependent changes in glutamate (Glu) and 7-aminobutyric acid ( GABA) release in the rostral ventromedial medulla in a rat model of incisional pain. Methods Healthy male SD rats weighing 250-300 g were used in this study. Twelve rats in which microdialysis cannulae were implanted in the right rostral ventromedial medulla without neurological deficits were randomly divided into 2 groups ( n = 6 each): group A control and group B incisional pain. In group B an 1 cm long incision was made in the plantar surface of right hindpaw under 1.2% isoflurane anesthesia which was maintained for 5 min. Samples of dialysate were collected before incision (T_0 baseline) and at 3 h, 1 d, 2 d and 3 d after incision was made (T_(1-4)) in both groups for determination of Clu and GABA concentrations (by HPLC). Results In group B Glu and GABA concentrations in the dialysate were significantly increased at 1 d (T_2) and 3 h-3 d (T_(1-4)) respectively as compared with the baseline value at T_0 and were significantly higher than those in group A (control group). Conclusion Incisional pain increases the release of Glu and GABA in the rostral ventromedial medulla which might influence the function of descending pain modulation pathway.
