Impact of elongation factor 1α expression change on proliferation and clone formation of prostate cancer cell line DU145
10.3760/cma.j.issn.1000-6702.2010.03.016
- VernacularTitle:前列腺癌DU145细胞增殖和克隆形成与延伸因子1α基因表达的关系
- Author:
Wei YAN
;
Weide ZHONG
;
Gang ZHU
;
Ben WAN
;
Jianye WANG
- Publication Type:Journal Article
- Keywords:
Peptide elongation factor 1;
RNA interference;
Prostatic neoplasms;
Cloning,molecular;
Cell proliferation
- From:
Chinese Journal of Urology
2010;31(3):203-206
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the elongation factor 1α(EF-1α) gene functions in prostate cancer cell line DU145 in the aspects of cell proliferation and clone formation by using the RNA interference technique. Methods DU145 cell lines were divided into control group, transfection control group transfected with scramble siRNA and experimental group transfected with EF-1α siRNA. After transfecting EF-1α siRNA into DU145 cell line, the down-regulation of EF-la expression in DU145 cell line was confirmed by Western blotting and immunofluorescence staining. Then, the cell proliferation and clone formation assays were carried on in these 3 groups of DU145 cells. Results Compared with controls, the specific down-regulation of EF-1α expression was achieved in experimental group only. Compared with control group, after the down-regualtion of EF-1α in DU145 cell line, the cell proliferation rate decreased from day 4 to day 7 after transfection by 45. 9%, 53. 5% , 35. 3% and 38. 1% , respectively(P<0. 05). The clone formation number in experimental group decreased by 67.0% (P<0. 01). Conclusions The down-regulation of EF-1α has a negative impact on prostate cancer cell proliferation and clone formation. EF-1α might be an appropiate targeting gene in prostate cancer targeting therapy.
