The immunoregulation of andrographolide on peripheral blood mononuclear cells from patients with chronic hepatitis B
10.3760/cma.j.issn.1000-6680.2010.04.008
- VernacularTitle:穿心莲内酯对慢性乙型肝炎患者外周血单个核细胞的免疫调节作用
- Author:
Feifei YANG
;
Wei WANG
;
Xinyan LI
;
Chunhong LIU
;
Di QU
;
Jiming ZHANG
;
Yuxian HUANG
- Publication Type:Journal Article
- Keywords:
Andrographolide;
Hepatitis B,chronic;
Monocytes;
Thl cells;
Th2 cells;
Interleukin-4;
lnterleukin-10
- From:Chinese Journal of Infectious Diseases
2010;28(4):226-231
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of andrographolide on the expressions of Th1 cytokine [interferon (IFN)-γ] mRNA and Th2 cytokines [interleukin (ID-4, IL-10] mRNA of peripheral blood mononuclear cells (PBMCs) from patients with chronic hepatitis B (CHB) and its anti-hepatitis B virus (HBV) activity. Methods PBMCs from CHB patients were cultured with 10 mg/L andrographolide (experimental group) or 0.1% DMSO (control group). HepG2. 2. 15 cells were stimulated with andrographolide of different concentrations (experimental group) or adefovir (control group). The expressions of IFN-γ mRNA, IL-4 mRNA and IL-10 mRNA of PBMCs and the replication of HBV DNA in HepG2. 2. 15 cell line were detected by fluorescence quantitative PCR. Results The expression of IFN-γ mRNA in PBMCs cultured with 10 mg/L andrographolide for 16 h was higher than that in control group (Z=-2. 78, P=0. 05), and the expressions of IL-4 and IL-10mRNA in experimental group were lower than control group (Z= -3. 82, P<0. 01), while the ratio of IFN-γ/IL-4 mRNA was higher than control group (Z= - 3. 82, P<0. 01). Andrographolide with different concentrations had no effect on the replication of HBV DNA in HepG2. 2.15 cells ((=11. 88, P>0.05). However, adefovir had inhibitory effect on the replication of HBV DNA (t =15. 95,P< 0. 05). Conclusion Andrographolide can regulate the expressions of IFN-γ mRNA, IL-4 mRNA and IL-10 mRNA in PBMCs from CHB patients and improve Thl/Th2 balance, while it has no effect on the replication of HBV DNA.
