Effect of Ganoderma lucidum on drug resistance of cisplantin-resistant ovarian cancer cell line

VernacularTitle:灵芝孢子对人卵巢癌顺铂耐药细胞株耐药性的影响
Author: Sufen ZHAO ; Yanjing WU ; Liyun CHEN ; Jianxin CHENG ; Zhenming DONG
Publication Type:Journal Article
Keywords: Ganoderma lueidum; Drug tolerance; Ovarian neoplasmas
From:Chinese Journal of Anesthesiology 2010;30(4):421-423
CountryChina
Language:Chinese
Abstract: Objective To evaluate the effect of Ganoderma lucidum on drug resistance of the cisplantin-resistant ovarian cancer cell line(A2780CP).Methods The A2780CP cells were randomly divided into control group and test group.The cells in control group and test group were incubated in culture media alone and in culture media containing Ganoderma lucidum 0.5 mg/ml for 48 h respectively.The resistance index(RI) of A2780CP was determined by WST-1 assay.The expression of Akt,Bcl-2 and p53 protein was measured by Western blot.Results The RI was significantly deeressed,the expression of Akt and Bcl-2 protein was down-regulated,while p53 protein expression was up-regulated in test group as compared with control group(P<0.05).Conclusion Ganoderma lueidum can reduce the resistance of A2780 CP cells to cisplantin by down-regulating the expression of Akt and Bcl-2 and up-regulating p53 expression in A2780CP cells.