The olfactory ensheathing cells transplantation for traumatic brain injury recovery of nerve function and its mechanism
10.3760/cma.j.issn.1674-6554.2010.07.013
- VernacularTitle:嗅鞘细胞移植促进脑损伤大鼠神经功能恢复及其作用机制研究
- Author:
Jian WANG
;
Qiqin DAN
;
Yu DUAN
;
Shanshan CHEN
;
Xiaojing LI
;
Tinghua WANG
;
Xue ZHOU
- Publication Type:Journal Article
- Keywords:
Olfactory ensheathing cells;
Traumatic brain injury;
Cell transplantation;
Neurological function recovery;
Neuronal survival
- From:Chinese Journal of Behavioral Medicine and Brain Science
2010;19(7):614-616
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe olfactory ensheathing cells transplantation on brain injury recovery nerve function and to explore its mechanism. Methods After purification of the olfactory ensheathing cells cultured for NGFRp75 immunocytochemical identification and preparation of cell suspension for transplantation, some cells were pre-labeled for bservation of survival after transplantation. 48 adult SD rats were randomly divided into three groups:sham operation without injury group (A group),cerebral cortex motor area injury group (B group) , the same brain injury and the olfactory ensheathing cell transplantation group (C group). At postoperative day,3 d, 7 d,14 d the neurological severity score (NSS) of rats were assessed; 14 d after injury of brain tissues were taken for NeuN immunohistochemistry host the number of neurons change. The data were statistically analyzed using SPSS17.0 software. Results (1) Cultured olfactory ensheathing cells showed NCFRp75 positive,the positive rate was 90%. (2) 14 d after transplantation of nuclear fluorescence labeling of olfactory ensheathing cells survived well in the host body. (3) 14 d after NSS score of B group( 2.00 ± 0.53) and C group ( 1.25 ± 0.46) were significantly better than the B group (P<0.05). (4) NeuN positive cells in B group (39.2 ±7. 1) and C group(45, 8 ± 6.0) were significantly better than B group (P<0.05). Conclusions Olfactory ensheathing cell transplantation can promote the recovery of neurological function in rats brain injury,which may be related with olfactory ensheathing cells to promote neuron survival in the host.
