Effect of intrathecal glial cell line-derived neurotrophic factor on p38MAPK protein expression in spinal dorsal horn in a rat model of neuropathic pain
10.3760/cma.j.issn.0254-1416.2010.05.015
- VernacularTitle:鞘内注射GDNF对神经病理性痛大鼠脊髓背角p38MAPK蛋白表达的影响
- Author:
Donglin JIA
;
Xinmin WU
;
Xiangyang GUO
;
Liping ZHANG
;
Jianrong GUO
- Publication Type:Journal Article
- Keywords:
Glial cell line-derived neurotrophic factor;
Injections,spinal;
Neuralgia;
p38 mitogen-activated protein kinases;
Spinal cord
- From:
Chinese Journal of Anesthesiology
2010;30(5):559-561
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of intrathecal glial cell line-derived neurotrophic factor (GDNF) on p38 mitogen-activated protein kinase (p38MAPK) protein expression in the spinal cord dorsal horn in a rat model of neuropathic pain. Methods Male SD rats 6 weeks old weighing 180-200 g were used in this study.One hundred and twenty rats in which intrathecal catheters was successfully implanted were randomly divided into 4 groups ( n = 30 each): group Ⅰ control ( group C); group Ⅱ sham operation ( group S); group Ⅲ neuropathicpain (group P) and group Ⅳ GDNF. In groupⅢ and Ⅳ I6 spinal nerve was ligated. In group Ⅳ intrathecal GDNF was administered every other day for 14 d after spinal nerve ligation. In group Ⅲ normal saline was given instead. Ten animals from each group were selected at 3, 7 and 14 d after spinal nerve ligation, the mechanical pain threshold was measured, and then the rats were decapitated. The I4-6 segment of the spinal cord of the operated side was isolated. p38MAPK protein expression in the spinul dorsal horn was determined by immunohistochemistry and Western blotting. Results Intrathecal GDNF significantly attenuated spinal nerve ligationinduced increase in p38MAPK protein expression in spinal dorsal horn of the operated side. Conclusion IT GDNF can relieve neuropathic pain by inhibiting p38MAPK protein expression in the spinal dorsal horn.
