The effect of the JAK/STAT signal pathway on apoptosis-related gene in the synovial tissue of rat rheumatoid arthritis
10.3760/cma.j.issn.1007-7480.2011.02.011
- VernacularTitle:Janus激酶/信号转导子与转录激活子通路对类风湿关节炎大鼠滑膜细胞凋亡相关基因表达的影响
- Author:
Wei GAO
;
Hui SHEN
;
Lijuan ZHAO
;
Jin LU
;
Weiguo XIAO
- Publication Type:Journal Article
- Keywords:
Arthritis,rheumatoid;
Apoptosis;
Janus kinase signal transducers and activators of transcription;
AG490
- From:Chinese Journal of Rheumatology
2011;15(2):111-115
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of the JAK/STAT signal pathway on the apoptosis-related gene in the synovial tissue of rat rheumatoid arthritis (RA).Methods Fifty rat models of collagen-induced arthritis,whose arthritis index was more than 2,were divided into the model group,the low dose of AG490 group,the medium dose of AG490 group and the high dose of AG490 group.In addition,6 rats were treated intraperitoneal injection.Then,the arthritis index and the change of apoptosis-related genes were compared.Multiple-sample average was analyzed by single-factor x2 test and LSD-t or Tamhane's T 2 test were used for two-two comparison.Results The arthritis index of the model group increased evidently,and the apoptosis inhibitor Bcl-2,Bcl-xl gene and protein expression was up-regulated,which was significantly different when compared with that of the control group(0.931±0.035 vs 0.351±0.024,0.920±0.037 vs 0.271±0.029,0.322±0.047 vs 0.230±0.031 ).The expression of apoptosis promoting factor Bax wasslightly up-regulated.The blockage of JAK/STAT pathway cotld down-regulate the expression levels of the gene and protein of survivins Bcl-2 and Bcl-xl,and up-regulate the gene and protein expressions of Bax.Conclusion In the process of RA development,apoptosis inhibitor Bcl-2,Bcl-xl gene and protein expression is up-regulated.JAK/STAT signal transduction pathway regulates the apoptosis process.
