Screening of genes associated with skin photoaging by microarray analysis
10.3760/cma.j.issn.0412-4030.2011.02.020
- VernacularTitle:应用基因芯片技术筛选皮肤光老化相关基因
- Author:
Wei YAN
;
Lili ZHANG
;
Shaohui LIU
;
Bingbing SHI
;
Zhenmin ZHAO
;
Dunmei WANG
;
Ningbei YIN
- Publication Type:Journal Article
- Keywords:
Skin aging;
Oligonucleotide array sequence analysis;
Differentially expressed genes
- From:
Chinese Journal of Dermatology
2011;44(2):130-133
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect differentially expressed genes between human normal skin and photoaging skin, and to investigate the molecular and biological mechanisms of human skin photoaging at transcriptional level. Methods Full-thickness skin specimens were obtained during full-face rhytidectomy from sun-exposed (anterior ear skin) and sun-protected (retroauricular skin) sites of 6 patients with facial photoaging from 2007 to 2008. Genomic microarray analysis was conducted to identify differentially expressed genes between the two groups of specimens followed by gene-cluster analysis. Results The normalization of microarray data showed that the number of differentially expressed genes was 2163 between skin samples from sun-exposed and sun-protected sites in one patient, significantly higher than that in the other 5 patients (less than 200);therefore, the data from the patient with 2163 differentially expressed genes were excluded from further analysis.Totally, 172 differentially expressed genes were identified with Beadarray chip, including 99 up-regulated genes and 73 down-regulated genes. Based on Genebank research, 118 functionally classified genes werefound, which were associated with a series of biological processes, including cell adhesion, receptor regulation,signal transduction, metabolism, and so on. Conclusions There are a lot of differentially expressed genes between human photoaging skin and normal skin. Rhytidectomy may be associated with the differential expression of skin photoaging-related genes.
