Experimental study of berberine anti macrophages apoptosis induced by Tg and Fuc

VernacularTitle:黄连素抗Tg和Fuc诱导的小鼠巨噬细胞凋亡的实验研究
Author: Liming LU ; Zaoyuan KUANG ; Zhenliang LUO ; Jietao LIN ; Xiaoyu CHEN ; Jingchun ZENG
Publication Type:Journal Article
Keywords: Bererine; Macrophages apoptosis; Atherosclerosis
From: Chinese Journal of Microbiology and Immunology 2009;29(12):1065-1068
CountryChina
Language:Chinese
Abstract: Objective To investigate the effect of berberine anti macrophages apoptosis, initially to determine the concentration of anti-apoptosis and to explore its mechanisms of treatment in atherosclerosis (AS). Methods Apoptosis of RAW264.7 macrophages is induced by 0.5 μmol/L Tg and 50 μg/ml Fuc. The cell survival rate and apoptosis rate was detected by MIT and flow cytometry, respectively. Results There was statistical significance between the berberine group of 800 μg/ml and normal group in the cell sur-vival rate(P<0.01). With the decline in concentration of berberine, the cell apoptosis rate showed a down-ward trend, berberine (0.02-0.63 μg/ml) effectively inhibited the cell apoptosis induced by Tg and Fuc. Conclusion Berberine group has little impact on the cell proliferation. Berberine can effectively inhibit the cell apoptosis induced by Tg and Fuc.