Effect of rapamycin on the proliferation and apoptosis of glomerular mesangial cells
10.3760/cma.j.issn.1001-7097.2009.11.008
- VernacularTitle:雷帕霉素对肾小球系膜细胞增殖及凋亡的影响
- Author:
Bo FU
;
Sifang ZHANG
;
Li ZHUO
;
Guangyan CAI
;
Yuansheng XIE
;
Ribao WEI
;
Qiang MA
;
Xiangmei CHEN
- Publication Type:Journal Article
- Keywords:
Sirolimus;
Mesangial ceils;
Cell proliferation;
Apoptosis
- From:Chinese Journal of Nephrology
2009;25(11):849-852
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of different concentrations of rapamycin on the proliferation and apoptosis of glomerular mesangial cells(GMCs)and to investigate the mechanism. Methods GMCs were treated with different concentrations of rapamycin(1 μg/L,2 μg/L,4 μg/L,8 μg/L,16 μg/L).After treatment for 24 h,48 h and 72 h,cell proliferation was assessed bv MTT colorimetric assay and the growth curve was traced.After treatment for 72 h,the cell cycle distribution and the apoptotic rate of GMCs in different concentrations of rapamycin were analyzed bv flow cytometry.The effects of different concentrations of rapamycin on the mRNA and protein expression of p27 and p53 were detected by RT-PCR and Western blot respectivelyResult The low dose of rapamycin(1 μ/L)could signiticanfly inhibit the proliferation of GMCs and showed no effect on apoptosis.The high dose of rapamycin (8-16 μg/L)could significantly increase the apoptotic rate of GMCs.Rapamycin could increase the mRNA and protein expression of p27 and p53. Conclusion Rapamycin can inhibit GMCs proliferation and promote GMCs apoptosis by increasing the expression of p27 and p53.
