Safety Evaluation of Methoxyflavones Mixture (Ⅰ)from Kempferia parviflora; 28-day Repeated Dose Oral Toxicity Test and Mouse Micronucleus Test
10.1625/jcam.12.79
- VernacularTitle:黒ショウガ由来メトキシフラボン混合物(Ⅰ)の安全性評価；マウスによる28日間反復経口投与試験・小核試験
- Author:
Hirotaka OHKUWA-HAYASHI
;
Takanori FUJITA
;
Takuya KAWATA
;
Yoshihisa NAKANO
;
Tomihisa OHTA
- Publication Type:Journal Article
- Keywords:
Kaempferia parviflora, methoxyflavone, 28-day repeated dose of oral toxicity test, mouse micronucleus test
- From:Japanese Journal of Complementary and Alternative Medicine
2015;12(2):79-85
- CountryJapan
- Language:Japanese
-
Abstract:
Kaempferia parviflora rhizome is used in traditional folk medicines for the treatments of various symptoms in Thailand since ancient times. Several types of methoxyflavones were identified from that plant and the functions of some of those were reported. We determined that five kinds of methoxyflavones (5-hydroxy-3,7,3’,4’-tetramethoxyflavone, 5-hydroxy-7-methoxyflavone, 5-hydroxy-3,7-dimethoxyflavone, 5-hydroxy-3,7,4’-trimethoxyflavone, 5-hydroxy-7,4’-dimethoxyflavone) were included the following treatments of K. parviflora rhizome. The 80 %ethanol extract of that were adsorbed resin, removed 70 % ethanol elution and the rest adsorbed materials were eluted with 99.5 % ethanol. The safety of that five methoxyflavones mixture was evaluated. We performed a 28-day repeated dose of oral toxicity test and a mouse micronucleus test. The former results showed no significant toxicity on body weight, blood analyses, organ weight, blood biochemical analyses. The latter results showed negative, believed that the sample has no mutagenicity for living bodies.
