Determination of twelve active compounds in Qili Qiangxin capsules by UPLC-MS.
- Author:
Ying LIU
;
Yue OUYANG
;
Song LI
;
Min-Yan LIU
;
Li QIAO
;
Shao-Hua ZHAO
- Publication Type:Journal Article
- MeSH:
Capsules;
chemistry;
Chromatography, High Pressure Liquid;
methods;
Drugs, Chinese Herbal;
chemistry;
Quality Control;
Spectrometry, Mass, Electrospray Ionization;
methods;
Tandem Mass Spectrometry;
methods
- From:
China Journal of Chinese Materia Medica
2014;39(10):1822-1825
- CountryChina
- Language:Chinese
-
Abstract:
In order to establish an UPLC-MS method for determination of twelve active compounds in Qili Qiangxin capsules including astragaloside, calycosin-7-0-glucoside, ginsenoside Rb1, ginsenoside Re, ginsenoside Rd, ginsenoside Rg1, ginsenoside Rf, periplocin, periplocoside H1, hesperidin, narirutin, isoquercitrin, the chromatographic separations were performedon a Phenomenex UPLC Kinetex C18 column (2.1 mm x 100 mm, 2.6 microm) with gradient elution of acetonitrile and 0.1% aqueous formic acidat a flow rate of 0.4 mL x min(-1). The temperature was set as 40 degrees C and injection volume was 5 microL. The monitoring of all analytes was achieved under the negative ionization mode with TOF-MS and TOF-MS/MS method. The twelve analytes showed good linearity (R2 > 0.9990) within the test ranges, the average recoveries were 98.0%-102%, respectively, and the RSD were less than 3.9%, respectively. The established method is simple, rapid, and sensitive, and can be used for quality control of Qili Qiangxin capsules.
