Chemical Constituents Identified from Fruit Body of Cordyceps bassiana and Their Anti-Inflammatory Activity.
10.4062/biomolther.2016.063
- Author:
Wonse SUH
1
;
Gyeongsug NAM
;
Woo Seok YANG
;
Gi Ho SUNG
;
Sang Hee SHIM
;
Jae Youl CHO
Author Information
1. School of Biotechnology, Yeungnam University, Gyeongsan 38541, Republic of Korea.
- Publication Type:Original Article
- Keywords:
Cordyceps bassiana;
Phytochemical study;
1,9-dimethylguanine;
NF-κB;
AP-1;
Anti-inflammatory activity
- MeSH:
Adenosine;
Agaricales;
Animals;
Chromatography;
Chromatography, High Pressure Liquid;
Cordyceps*;
Dermatitis;
Dermatitis, Atopic;
Fruit*;
Genes, Reporter;
Luciferases;
Mannitol;
Mice;
Niacinamide;
Prostaglandin-Endoperoxide Synthases;
RNA, Messenger;
Transcription Factor AP-1;
Tumor Necrosis Factor-alpha;
Uridine
- From:Biomolecules & Therapeutics
2017;25(2):165-170
- CountryRepublic of Korea
- Language:English
-
Abstract:
Cordyceps bassiana is one of Cordyceps species with anti-oxidative, anti-cancer, anti-inflammatory, anti-diabetic, anti-obesity, anti-angiogenic, and anti-nociceptive activities. This mushroom has recently demonstrated to have an ability to reduce 2,4-dinitrofluorobenzene-induced atopic dermatitis symptoms in NC/Nga mice. In this study, we further examined phytochemical properties of this mushroom by column chromatography and HPLC analysis. By chromatographic separation and spectroscopic analysis, 8 compounds, such as 1,9-dimethylguanine (1), adenosine (2), uridine (3), nicotinamide (4), 3-methyluracil (5), 1,7-dimethylxanthine (6), nudifloric acid (7), and mannitol (8) were identified from 6 different fractions and 4 more subfractions. Through evaluation of their anti-inflammatory activities using reporter gene assay and mRNA analysis, compound 1 was found to block luciferase activity induced by NF-κB and AP-1, suppress the mRNA levels of cyclooxygenase (COX)-2 and tumor necrosis factor (TNF)-α. Therefore, our data strongly suggests that compound 1 acts as one of major principles in Cordyceps bassiana with anti-inflammatory and anti-atopic dermatitis activities.
