Calcium Signaling of Lysophosphatidylethanolamine through LPA1 in Human SH-SY5Y Neuroblastoma Cells.
10.4062/biomolther.2016.046
- Author:
Jung Min LEE
1
;
Soo Jin PARK
;
Dong Soon IM
Author Information
1. Molecular Inflammation Research Center for Aging Intervention (MRCA) and College of Pharmacy, Pusan National University, Busan 46241, Republic of Korea. imds@pusan.ac.kr
- Publication Type:Original Article
- Keywords:
Lysophosphatidylethanolamine;
LPA₁;
Lysophosphatidic acid;
GPCR;
Neuroblastoma;
Receptor
- MeSH:
Calcium Signaling*;
Calcium*;
Cell Membrane;
GTP-Binding Proteins;
Humans*;
Neuroblastoma*;
Ovarian Neoplasms
- From:Biomolecules & Therapeutics
2017;25(2):194-201
- CountryRepublic of Korea
- Language:English
-
Abstract:
Lysophosphatidylethanolamine (LPE), a lyso-type metabolite of phosphatidylethanolamine, has been reported to be an intercellular signaling molecule. LPE mobilizes intracellular Ca²⁺ through G-protein-coupled receptor (GPCR) in some cells types. However, GPCRs for lysophosphatidic acid (LPA) were not implicated in the LPE-mediated activities in LPA GPCR overexpression systems or in SK-OV3 ovarian cancer cells. In the present study, in human SH-SY5Y neuroblastoma cells, experiments with LPA₁ antagonists showed LPE induced intracellular Ca²⁺ increases in an LPA₁ GPCR-dependent manner. Furthermore, LPE increased intracellular Ca²⁺ through pertussis-sensitive G proteins, edelfosine-sensitive-phospholipase C, 2-APB-sensitive IP₃ receptors, Ca²⁺ release from intracellular Ca²⁺ stores, and subsequent Ca²⁺ influx across plasma membranes, and LPA acted on LPA₁ and LPA₂ receptors to induce Ca²⁺ response in a 2-APB-sensitive and insensitive manner. These findings suggest novel involvements for LPE and LPA in calcium signaling in human SH-SY5Y neuroblastoma cells.
