Protective effect of 3,4-oxo-isopropylidene-shikimic acid on vascular endothelial cell injured by hydrogen peroxide.
- Author:
Yi MA
1
;
Jian-ning SUN
;
Qiu-ping XU
;
Ya-jian GUO
Author Information
- Publication Type:Journal Article
- MeSH: 6-Ketoprostaglandin F1 alpha; metabolism; Cell Survival; drug effects; Cells, Cultured; Endothelial Cells; metabolism; pathology; Humans; Hydrogen Peroxide; antagonists & inhibitors; Infant, Newborn; L-Lactate Dehydrogenase; metabolism; Nitric Oxide; metabolism; Protective Agents; pharmacology; Shikimic Acid; analogs & derivatives; pharmacology; Umbilical Veins; cytology
- From: Acta Pharmaceutica Sinica 2003;38(12):897-899
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo study the effect of 3,4-oxo-isopropylidene-shikimic acid (ISA) on H2O2 (200 mol.L-1, 4 h) injured human umbilical vein endothelial cells (HUVEC).
METHODSMorphological change was observed under microscop. Cell viability was assessed by MTT assay. The release of intracellular lactate dehydrogenase (LDH) and NO was assessed by colorimetry. Radioimmunoassay was used to assess 6-keto-prostaglandin F1 alpha (6-keto-PGF1 alpha).
RESULTSPretreatment with ISA for 6 h alleviated the morphological damage of H2O2 induced HUVECs. At the concentration of 1-100 mumol.L-1, ISA prevented the inhibitory effect on cell viability induced by H2O2 in dose-dependent manner, but increased the ratio of cell viability from 60.4% to 84.3%. ISA reduced LDH release and increased the level of NO and 6-keto-PGF1 alpha in H2O2 induced HUVECs.
CONCLUSIONISA exerted protective effect on H2O2 injured HUVEC.
