CyPA-CD147-ERK1/2-cyclin D2 signaling pathway is upregulated during rat left ventricular hypertrophy.
- Author:
Fu-Cai TANG
1
;
Hong-Yan WANG
2
;
Ming-Ming MA
3
;
Tian-Wang GUAN
1
;
Long PAN
1
;
Dun-Chen YAO
1
;
Ya-Lan CHEN
1
;
Wei-Bei CHEN
1
;
Yong-Sheng TU
4
;
Xiao-Dong FU
1
Author Information
1. Department of Physiology, School of Basic Sciences, Guangzhou Medical University, Guangzhou 510182, China.
2. Department of Pathology, School of Basic Sciences, Guangzhou Medical University, Guangzhou 510182, China.
3. Department of Pharmacology, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou 510080, China.
4. Department of Physiology, School of Basic Sciences, Guangzhou Medical University, Guangzhou 510182, China. tuys@gzhmu.edu.cn.
- Publication Type:Journal Article
- MeSH:
Animals;
Basigin;
metabolism;
Blood Pressure;
Cyclin D2;
Cyclophilin A;
metabolism;
Hypertension;
Hypertrophy, Left Ventricular;
metabolism;
Mitogen-Activated Protein Kinase 1;
metabolism;
Mitogen-Activated Protein Kinase 3;
metabolism;
Myocytes, Cardiac;
Rats;
Rats, Sprague-Dawley;
Signal Transduction;
Up-Regulation
- From:
Acta Physiologica Sinica
2015;67(4):393-400
- CountryChina
- Language:English
-
Abstract:
The changes of serum cyclophilin A (CyPA), its receptor CD147 and the downstream signaling pathway during the process of cardiac hypertrophy remain unknown. The present study aims to investigate the relationships between CyPA-CD147-ERK1/2-cyclin D2 signaling pathway and the development of cardiac hypertrophy. Left ventricular hypertrophy was prepared by 2-kidney, 2-clip in Sprague-Dawley rats and observed for 1 week, 4 and 8 weeks. Left ventricular hypertrophy was evaluated by ratio of left ventricular heart weight to body weight (LVW/BW) and cardiomyocyte cross sectional area (CSA). CyPA levels in serum were determined with a rat CyPA ELISA kit. Expressions of CyPA, CD147, phospho-ERK1/2 and cyclin D2 in left ventricular myocytes were determined by Western blot and immunostaining. Compared with sham groups, systolic blood pressure reached hypertensive levels at 4 weeks in 2K2C groups. LVW/BW and CSA in 2K2C groups were significantly increased at 4 and 8 weeks after clipping. ELISA results indicated a prominent increase in serum CyPA level associated with the degree of left ventricular hypertrophy. Western blot revealed that the expressions of CyPA, CD147, phospho-ERK1/2 and cyclin D2 in left ventricular tissues were also remarkably increased as the cardiac hypertrophy developed. The results of the present study demonstrates that serum CyPA and CyPA-CD147-ERK1/2-cyclin D2 signaling pathway in ventricular tissues are time-dependently upregulated and activated with the process of left ventricular hypertrophy. These data suggest that CyPA-CD147 signaling cascade might play a role in the pathogenesis of left ventricular hypertrophy, and CyPA might be a prognosticator of the degree of left ventricular hypertrophy.
