Comparison of 3 Automated Immunoassays for Detection of Anti-Hepatitis A Virus Immunoglobulin M in a Tertiary Care Hospital.
10.3343/alm.2013.33.2.121
- Author:
Hyewon PARK
1
;
Yu Joo LEE
;
Moon Woo SEONG
;
Do Hoon LEE
;
Myoung Hee PARK
;
Eun Young SONG
Author Information
1. Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Korea. eysong1@snu.ac.kr
- Publication Type:Brief Communication ; Comparative Study
- Keywords:
Anti-hepatitis A virus immunoglobulin M;
Immunoassay;
Agreement
- MeSH:
Hepatitis A virus/genetics/*immunology;
Hepatitis B/*diagnosis;
Hospitals, University;
Humans;
Immunoassay/*methods;
Immunoglobulin M/*analysis;
RNA, Viral/analysis;
Reagent Kits, Diagnostic;
Retrospective Studies;
Reverse Transcriptase Polymerase Chain Reaction;
Tertiary Healthcare
- From:Annals of Laboratory Medicine
2013;33(2):121-124
- CountryRepublic of Korea
- Language:English
-
Abstract:
Three automated immunoassay kits for anti-Hepatitis A Virus (HAV) IgM-Architect, (Abbott Laboratories, USA), Elecsys (Roche Diagnostics, Germany), and ADVIA Centaur (Siemens Healthcare Diagnostics Inc., USA)-were compared. We included 178 consecutive samples, for which an anti-HAV IgM test was requested at Seoul National University Hospital from September 2009 to January 2010. Reviewing of medical records, reverse transcription (RT)-PCR for HAV RNA, or total anti-HAV assay were performed on 16 (9.0%) samples with discrepant results. The percent agreements (kappas) of the Architect and ADVIA Centaur, Architect and Elecsys, and ADVIA Centaur and Elecsys kits were 96.6% (0.91), 96.6% (0.92), and 97.8% (0.94), respectively. Eight out of 16 discrepant samples showed gray-zone values in Architect but were nonreactive in the others. Slightly earlier seroconversion was suspected in Elecsys. The 3 assays showed comparable performances with excellent agreements in a tertiary care hospital setting.
