Tumor-associated proteins in rat submandibular gland induced by DMBA and irradiation.
- Author:
Sung Ook OH
1
;
Son Chul CHOI
;
Tae Won PARK
;
Dong Soo YOU
Author Information
1. Department of Oral and Maxillofacial Radiology, College of Dentistry, Seoul National University, Korea.
- Publication Type:Original Article
- Keywords:
Irradiation;
DMNA;
Tumor associated membrane proteins;
Immunoelectrophoresis;
Double immunodiffusion;
Tumor specific antigen
- MeSH:
9,10-Dimethyl-1,2-benzanthracene*;
Adult;
Animals;
Cell Membrane;
Electrophoresis, Polyacrylamide Gel;
Heart;
Humans;
Immune Sera;
Immunodiffusion;
Immunoelectrophoresis;
Liver;
Membrane Proteins;
Molecular Weight;
Pancreas;
Rats*;
Skin;
Staphylococcal Protein A;
Submandibular Gland*
- From:Journal of Korean Academy of Oral and Maxillofacial Radiology
1997;27(2):63-82
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
This study was performed in order to identify changes of the plasma membrane proteins in rat submandibular gland tumors induced by 7,12-dimethylbenz[a]anthracene [DMBA] and X-irradiation. Two kinds of tumor associated membrane proteins (protein A and B) were isolated with 3 M KCl extraction from rat submandibular gland tumors induced by DMBA and X-irradiation. To identify their antigenicities, immunoelectrophoresis and double immunodiffusion was carried out with various proteins extracted from liver, heart, skin and pancreas of adult rats and from embryonic liver, heart and skin. The rabbit antisera against the protein A did not cross-react with any of the proteins extracted from the above mentioned tissues, suggesting that protein A might be tumor specific antigen. However, the rabbit antisera against protein B was precipitated with proteins extracted from the liver of adult and embryonic rats. Polyacrylamide gel electrophoresis of these two proteins (A and B) showed that protein A was a dimer with molecular weights of 69,000 and 35,000 dalton, whereas protein B was a monomer with molecular weight of 50,000 dalton.
