Experimental Study on the Radiosensitivity and Chemosensitivity of A-431 Cell Line.
- Author:
Seong Woo HONG
1
;
Eun suk CHOI
;
Kwang Joon KOH
Author Information
1. Department of Oral and Maxillofacial Radiology, School of Dentistry, Chonbuk National University, Korea.
- Publication Type:Original Article
- Keywords:
radiosensitivity;
chemosensitivity
- MeSH:
Bleomycin;
Carcinoma, Squamous Cell;
Cell Line*;
Cisplatin;
Drug Therapy;
Humans;
Radiation Tolerance*;
Radiotherapy
- From:Journal of Korean Academy of Oral and Maxillofacial Radiology
1999;29(1):327-339
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVES: The purpose of this study was to aid in the prediction of tumor cell tolerance to radiotherapy and/or chemotherapy. MATERIALS AND METHODS: Human epidermoid carcinoma A-431 cell line were irradiated by 2, 4, 6, 8, 10Gy at a dose rate of 210cGy/min using 60Co Irradiator ALDORADO 8 and then were exposed to bleomycin or cisplatin at concentration of 2mug/mul for 1 hour. The viable cells were determined for each radiation dose and/or each drug at the 4th day and cell surviving curves were obtained using semiautomated MTT assay. RESULTS: The surviving fraction after irradiation of 2Gy was 0.99, and there was not significant difference of surviving fraction in comparison with the control group on A-431 cell line(p>0.05). But there were significant differences of surviving fractions at doses of 4, 6, 8, 10Gy in comparison with the control group(p<0.05). The cytotoxicity of bleomycin or cisplatin was significantly different in comparison with the control group on A-431 cell line (p<0.05). And the cytotoxicity of cisplatin was greater than that of bleomycin on A-431 cell line (p<0.05). There were significant differences of surviving fractions after irradiation of 2, 4, 6, 8, 10Gy with bleomycin or cisplatin in comparison with each group of irradiation only on A-431 cell line(p<0.05). There were significant differences of surviving fractions between the groups of irradiation with bleomycin and cisplatin at doses of 2, 4Gy(p<0.05), but there were not significant differences of surviving fractions at doses of 6, 8, 10Gy on A-431 cell line (p>0.05).
