Tissue localization of insulin-like peptide in Haemonchus contortus and identifica-tion and expression of its encoding genes
10.16303/j.cnki.1005-4545.2024.06.13
- VernacularTitle:捻转血矛线虫胰岛素样肽编码基因的鉴定、表达与组织定位
- Author:
Wei LIU
1
;
Fei WU
;
Xueqiu CHEN
;
Zhendong DU
;
Guangxu MA
;
Aifang DU
Author Information
1. 浙江大学 动物科学学院 动物预防医学研究所 浙江省动物预防医学重点实验室,浙江杭州 310058
- Keywords:
Haemonchus contortus;
insulin-like peptides;
prokaryotic expression;
tissue localization;
developmental regulation;
larval diapause
- From:
Chinese Journal of Veterinary Science
2024;44(6):1184-1193
- CountryChina
- Language:Chinese
-
Abstract:
Insulin-like peptides(ILPs)play a crucial role in the biological processes of nematode metabolism,signal transduction,and developmental processes,representing potential therapeutic targets for nematode infections in animals.In order to explore the specific role of ILPs in nematode development and infection,genome-wide identification,sequence and evolution analysis of the ILPs coding genes were conducted on Haemonchus contortus(H.contortus);transcriptional levels of these ILPs coding genes among developmental stages were determined by a real-time quantitative PCR method;recombinant ILPs were produced in prokaryotic system for polyclonal antibody prep-aration and Western blot analysis;indirect immunofluorescence localization experiment was used to reveal the tissue distribution of ILPs at different developmental stages.It was showed that the number of ILPs coding genes in H.contortus were significantly reduced compared with that of Caenorhabditis elegans,namely three members of ILPs coding genes exhibiting antagonistic fea-tures;the highest transcriptional levels of ILPs coding genes was detected in the infective third stage larvae of H.contortus;ILPs were found dominant in the intestine and hypodermis of the in-fective larvae of this parasitic nematode.In this study,three genes encoding antagonistic ILPs were identified in H.contortus,they might play a role in regulating the development and infection processes.The finding lay a foundation for the study of nematode hypobiosis(larval diapause)in animals and the screening of potential intervention targets.
