Effects of ginkgetin on local microvascular and nerve function defects during cerebral ischemia/reperfusion injury in rats
10.3969/j.issn.1000-4718.2024.07.014
- VernacularTitle:银杏双黄酮对大鼠脑缺血再灌注损伤时局部微血管及神经功能缺损的影响
- Author:
Chao CHEN
1
;
Guangqing CHENG
;
Changsheng LI
;
Aishuai WANG
;
Anrong WANG
;
Xiaoni YANG
Author Information
1. 山东第一医科大学第一附属医院(山东省千佛山医院),山东 济南 250014
- Keywords:
Ginkgetin;
Ischemic stroke;
Ischemia/reperfusion;
Neuroprotection;
Angiogenesis
- From:
Chinese Journal of Pathophysiology
2024;40(7):1261-1267
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the effects of ginkgetin on neurological deficit and angiogenesis induced by cerebral ischemia/reperfusion(I/R)and its underlying mechanism.METHODS:Sixty SD rats were randomly allocated into three groups:sham group,I/R model group,and ginkgetin(40 mg/kg)treatment(I/R+ginkgetin)group,with twenty rats in each group.The middle cerebral artery occlusion/reperfusion(MCAO/R)rat model was employed to simulate cere-bral I/R,and ginkgetin was administered continuously for 7 days following reperfusion.The cerebral infarction volume was quantified using TTC staining.Neuronal density in the ischemic penumbra was assessed through Nissl staining and immu-nohistochemistry for neuron-specific nuclear protein(NeuN).Microvessel density and angiogenesis in the ischemic pen-umbra of each group were analyzed using CD31 labeling and BrdU/von willebrand factor(vWF)double labeling immuno-fluorescence staining.Western blot analysis was performed to determine the levels of heat shock protein 70(HSP70),vas-cular endothelial growth factor(VEGF),and hypoxia inducible factor-1α(HIF-1α)in the ischemic penumbra.RE-SULTS:Compared with the I/R model group,the cerebral infarction volume was significantly reduced in ginkgetin treat-ment group(P<0.01),the number of neurons,the microvessel density,angiogenesis and the expression levels of HIF-1α,VEGF,and HSP70 in the ischemic penumbra were significantly increased(P<0.01).CONCLUSION:Ginkgetin exhibits the potential to augment angiogenesis and facilitate neurological function recovery in MCAO rats,while concur-rently upregulating the expression of HSP70,VEGF,and HIF-1α within the ischemic penumbra.
