Dose-dependent embryotrophic effect of recombinant granulocyte-macrophage colony-stimulating factor and brain-derived neurotrophic factor in culture medium for mouse preimplantation embryo.
10.5468/ogs.2014.57.5.373
- Author:
Jee Hyun KIM
1
;
Hyun Ju LEE
;
Eun Jeong YU
;
Byung Chul JEE
;
Chang Suk SUH
;
Seok Hyun KIM
Author Information
1. Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital, Seongnam, Korea. blasto@snubh.org
- Publication Type:In Vitro ; Original Article
- Keywords:
Brain-derived neurotrophic factor;
Culture medium;
Embryotrophic effects;
Granulocyte-macrophage colony-stimulating factor
- MeSH:
Animals;
Blastocyst*;
Brain-Derived Neurotrophic Factor*;
Cell Count;
Embryonic Structures;
Female;
Granulocyte-Macrophage Colony-Stimulating Factor*;
Humans;
Male;
Mice*;
Oocytes;
Spermatozoa
- From:Obstetrics & Gynecology Science
2014;57(5):373-378
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVE: To evaluate the dose effect of recombinant mouse granulocyte-macrophage colony-stimulating factor (rmGM-CSF) or brain-derived neurotrophic factor (BDNF) in culture medium on the development of in vitro fertilized mouse embryos. METHODS: Mature oocytes were retrieved from superovulated female BDF1 mice and inseminated by sperm from male BDF1 mice. On day 1, two-cell stage embryos were divided and cultured until day 5 in the embryo maintenance medium supplemented with 0, 1, 2, 5, or 10 ng/mL of rmGM-CSF or supplemented with 0, 5, 10, or 20 ng/mL of BDNF. Blastocyst formation rate and their cell numbers were assessed. RESULTS: The blastocyst formation rate and the total cell count in blastocyst was similar in all the rmGM-CSF treatment groups when compared with the control. However, the blastocyst formation rate and the total cell count was significantly higher in the group supplemented with 10 ng/mL of BDNF compared with the control (63.9%, 45.8+/-11.5 vs. 52.3%, 38.0+/-6.8; P<0.05, respectively). CONCLUSION: Supplementation of 10 ng/mL of BDNF enhanced the developmental potential of mouse preimplantation embryos, but supplementation of rmGM-CSF did not.
