Molecular Identification of Magnoliae Flos and Its Relatives Based on DNA Barcoding
10.14148/j.issn.1672-0482.2023.1086
- VernacularTitle:中药辛夷及其近缘种的DNA条形码分子鉴定
- Author:
Xin-Yi ZHANG
1
,
2
;
Yu-Xuan QIU
;
Yang HU
;
Rong TIAN
;
Mian ZHAO
;
Hui YAN
;
Xia-Chang WANG
;
Li-Hong HU
Author Information
1. 南京中医药大学江苏省中药功效物质重点实验室,江苏 南京 210023
2. 南京中医药大学药学院,江苏 南京 210023
- Keywords:
Magnoliae Flos;
DNA barcoding;
molecular identification;
psb A-trn H+mat K;
grafting reproduction
- From:
Journal of Nanjing University of Traditional Chinese Medicine
2023;39(11):1086-1094
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To identify Magnoliae Flos and its related species using DNA barcoding technology,so as to establish a rapid,accurate and convenient method for identification of Magnoliae Flos.METHODS Genomic DNA of 21 Magnoliae Flos and their related species were extracted,and ITS,ITS2,psb A-trn H,mat K and rbc L gene sequences were amplified and sequenced.MEGA7.0 software was used for sequence comparison,and the intraspecific and interspecific genetic distance was calculated based on Kimura-2-Parameter(K2P)model to evaluate Barcoding gap,and the NJ phylogenetic trees were constructed to react identification re-sults.RESULTS Among the 5 primers,psb A-trn H and mat K could normally amplify the target gene,and the success rate of them was higher than 98%,while the success rate of rbc L sequencing was 38.1%only.The mat K sequence had the highest number of varia-tion sites,and psb A-trn H had the most prominent species discriminatory on Barcoding gap analysis relative to mat K and rbc L.The NJ phylogenetic tree showed that psb A-trn H+mat K barcode sequence could accurately identify 9 varieties,and distinguish Magnoliae Flos and its relatives.In addition,we applied it to the molecular identification of Magnolia grafting rootstocks for the first time.CONCLU-SION The sequence combination of psb A-trn H+mat K can realize the accurate identification of Magnoliae Flos,and can be applied to the rapid identification of Magnolia grafting rootstocks.
