Dysregulated Production of IFN-g, IL-12, and IL-10 by Peripheral Blood Mononuclear Cells from Early Active Pulmonary and Multidrug-Resistant Tuberculosis Patients.
- Author:
Ji Sook LEE
1
;
Hee Sook SON
;
Chang Hwa SONG
;
Hwa Jung KIM
;
Jeong Kyu PARK
;
Tae Hyun PAIK
;
Ji Won SUHR
;
Chul Hee KIM
;
Suck Jun KONG
;
Mal Hyun SHON
;
Eun Kyeong JO
Author Information
1. Department of Microbiology, College of Medicine, Chungnam National University, 6 Munhwa-dong, Jung-ku, Taejon, Korea. hayoungj@cnu.ac.kr
- Publication Type:In Vitro ; Original Article
- Keywords:
Mycobacterium tuberculosis;
Interferon-gamma;
Interleukin-12;
Interleukin-10;
Multidrug-resistant tuberculosis
- MeSH:
Cytokines;
Enzyme-Linked Immunosorbent Assay;
Humans;
Interferon-gamma;
Interleukin-10*;
Interleukin-12*;
Mycobacterium tuberculosis;
RNA, Messenger;
Tuberculin;
Tuberculosis;
Tuberculosis, Multidrug-Resistant*;
Tuberculosis, Pulmonary
- From:Journal of Bacteriology and Virology
2002;32(2):211-220
- CountryRepublic of Korea
- Language:English
-
Abstract:
In this study, we investigated profiles of the cytokines IFN-g, IL-12, and IL-10 in active pulmonary tuberculosis (EAPTB) patients, HIV-negative patients with multidrug-resistant tuberculosis (MDR-TB) and in healthy tuberculin reactors (HTR). We studied the responses of peripheral blood mononuclear cells (PBMC) from 12 EAPTB patients and 15 MDR-TB patients to stimulation with a purified protein derivatives (PPD) antigen (Ag), and compared them with those from 14 HTR. Using ELISA, IFN-g production was found to be significantly depressed, while IL-10 was significantly elevated in both MDR-TB and EAPTB after in vitro stimulation with PPD, compared with those in HTR. Although there was no significant difference in IL-12 production among the three groups, mean IL-12 production was highest in patients with MDR-TB. In these patients, IL-12 production was significantly correlated with IL-10 expression, but not IFN-g production. In addition, neutralization of endogenous IL-10 led to enhanced IFN-g and IL-12Rb2 mRNA expression in TB patients. Our findings suggest that both groups of TB patients may have a similar disregulated pattern of IL-12, IL-10, and IFN-g production during M. tuberculosis infection. Furthermore, the results suggest a potentially pathogenic role for IL-10 in impaired Th1 immune responses in TB patients.
