Effect of thioridazine on the proliferation,migration and invasion of liver cancer Huh-7 cells by regulating miR-3174 expression
10.12173/j.issn.1008-049X.202310067
- VernacularTitle:硫利达嗪通过调控miR-3174表达对肝癌Huh-7细胞增殖、迁移和侵袭的影响
- Author:
Xiao-Dan ZHANG
1
;
Jiang-Wei SU
;
Jing LIANG
;
Ping LIANG
Author Information
1. 河北省胸科医院药学部(石家庄 050041)
- Keywords:
Thioridazine;
Liver cancer;
miR-3174;
Proliferation;
Migration;
Invasion
- From:
China Pharmacist
2023;26(11):189-196
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect and possible mechanism of thioridazine on the biological behavior of liver cancer Huh-7 cells.Methods Hepatocellular carcinoma Huh-7 cells were randomly divided into a control group and low,medium,and high concentration groups of thiazidine.The miR-3174 mimics and its negative control,miR-3174 inhibitor and its negative control were transfectel to Huh-7 cells,and the miR-3174 mimics and its negative control were treated with thiolidazine.Effect of thioridazine on the proliferation of Huh-7 cells was assessed by CCK-8 method and plate cloning experiment;effect of thioridazine on the migration and invasion of Huh-7 cells was calculated using scratch healing test and Transwell experiment;effect of thioridazine on the expression of miR-3174 in Huh-7 cells was detected by RT-qPCR;E-cadherin and N-cadherin proteins expression was analyzed using Western blotting.The effects of interference with miR-3174 or miR-3174 up-regulation combined with thioridazine on the biological behavior of Huh-7 cells were detected by the above methods.Results After thiolidazine treatment and interference with miR-3174 expression,miR-3174 expression in Huh-7 cells was decreased,proliferation,migration and invasion ability,N-cadherin protein expression were decreased,and E-cadherin protein expression was increased(P<0.05).Up-regulating the expression of miR-3174 attenuated the effect of thiolidazine treatment on the malignant biological behavior of Huh-7 cells.Conclusion Thioridazine inhibits the proliferation,migration and invasion of liver cancer Huh-7 cells by down-regulating miR-3174 expression.