A study on the animal model preparation and early histological changes of trans-sutural distraction osteo-genesis
10.3969/j.issn.1001-3733.2024.02.004
- VernacularTitle:经缝牵引成骨动物模型制备及早期组织学变化研究
- Author:
Zhihe ZHAO
1
;
Yufan ZHANG
;
Wenhui ZHANG
;
Yicheng CHEN
;
Xuelian JIA
;
Shanluo ZHOU
;
Fuding SHEN
;
Yuxuan DU
;
Yunpeng LI
;
Liang KONG
Author Information
1. 710032 西安,口颌系统重建与再生全国重点实验室,国家口腔疾病临床医学研究中心,陕西省口腔疾病临床医学研究中心,空军军医大学第三附属医院口腔颌面外科
- Keywords:
Trans-sutural distraction osteogenesis;
Animal model;
Early-stage change
- From:
Journal of Practical Stomatology
2024;40(2):173-179
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish an animal model of trans-sutural distraction osteogenesis in SD rats.Methods:A self-designed V-shaped distraction device(distractor)was fabricated with the traction force(N)of 0,1.3,2.2,3.0,4.3 and 5.0 corresponding to the distraction length(mm)of 5,4,3,2,1 and 0 respectively,meeting the trans-sutural distraction osteogenesis requirements in skull of 5-week-old SD rats.The distractor was plased into the sagittal suture of 12 SD rats.Continuous sampling was conducted 1,3,5 and 7 days respectively(n=3)after operation.The tissue changes in the trans-sutural distraction area were observed by HE and Masson's trichrome staining.Inflammation levels were determined using Arg-1 immunofluorescence staining.The early angiogenesis was clarified through co-staining with CD31 and EMCN.Results:A stable trans-sutural distraction osteogenesis model was estab-lished,5 mm distraction osteogenesis width was observed completely within 7 days of distraction.Significant new bone formation was observed at 7 days after operation.Arg-1 expression increased and was concentrated at the bone margins,overlapping with the areas of new bone formation.EMCN expression gradually decreased,and by day 7 CD31 was predominant,indicating the basic maturation of blood vessels.Conclusion:This study successfully constructed a stable and effective trans-sutural distraction osteogenesis animal model,and provides an experimental basis for the investigation of its early continuous histological changes.
