Expression of adiponectin receptor T-cadherin in acute myocardial infarction and its association with acute inflammatory reaction
10.3760/cma.j.issn.1671-0282.2023.08.014
- VernacularTitle:脂联素受体T-钙黏蛋白在急性心肌梗死中的表达及与急性炎症反应关联性研究
- Author:
Juanjuan WANG
1
;
Fengying CHEN
Author Information
1. 内蒙古医科大学附属医院急诊科，呼和浩特 010059
- Keywords:
T-cadherin;
Adiponectin;
Acute Myocardial Infarction;
Hypersensitive C-reactive protein;
Tumor Necrosis Factor-Alpha
- From:
Chinese Journal of Emergency Medicine
2023;32(8):1090-1095
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the expression of adiponectin receptor T-cadherin in ISO-induced rat myocardial infarction model and its association with acute inflammatory reaction.Methods:Twenty-four male Wistar rats were randomly divided into the myocardial infarction model group and the control group, with 12 rats in each group. After normal electrocardiogram recording, rats in the myocardial infarction model group were injected subcutaneously with isoproterenol (ISO) 150 mg·kg -1·d -1 for 2 consecutive days. The control group was subcutaneously injected with the same amount of normal saline for 2 consecutive days. Electrocardiogram was recorded 24 h after the second subcutaneous injection of ISO. After the success of the model, the rats were sacrificed, the heart tissue was stained with HE, and the protein expression of T-cadherin in the myocardium was detected by immunohistochemistry. The levels of adiponectin, T-cadherin, hypersensitive C-reactive protein (hS-CRP) and tumor necrosis factor alpha (TNF-α) in serum were detected by ELISA. Results:Immunohistochemical results showed that the protein expression of T-cadherin decreased in the myocardial infarction model group [(0.1567±0.0061) vs. (0.1228±0.0027)], and the difference was statistically significant ( P<0.05). ELISA showed that serum adiponectin (ng/mL) content in the myocardial infarction model group was significantly decreased [(7.016±0.7236) vs. (1.883±0.2240)], and the difference between the two groups was statistically significant ( P<0.01). Serum T-cadherin (ng/mL) [(6.075±0.8149) vs. (14.610±1.583)], serum TNF-α (pg/mL) [(1860±95.96) vs. (3141±92.5)] and serum hs-CRP (ng/mL) [(20.93±1.079) vs. (30.49±1.742)] in the myocardial infarction model group were significantly increased, and the differences between the two groups were statistically significant ( P<0.01). Serum T-cadherin was negatively correlated with serum adiponectin, and serum T-cadherin was positively correlated with inflammatory factors (TNF-α and hs-CRP), while serum adiponectin was negatively correlated with inflammatory factors (TNF-α and hs-CRP). Conclusions:T-cadherin is involved in the inflammatory response of acute myocardial infarction, which may be an important molecular marker of acute myocardial injury and play an important role in the disease early warning.
