Development of Digital Polymerase Chain Reaction Technology and Its Progress in Disease Detection
10.19756/j.issn.0253-3820.231323
- VernacularTitle:数字聚合酶链式反应技术及其在疾病检测中的研究进展
- Author:
Xu-Dong BAO
1
,
2
,
3
;
Xiao-Lin HU
;
Qi-Wu WAN
;
Hong ZHANG
;
Yang LUO
Author Information
1. 重庆大学医学院,智慧检验与分子医学中心,重庆 400030
2. 重庆大学生物工程学院,重庆 400044
3. 国家卫健委出生缺陷与生殖健康重点实验室,重庆 400020
- Keywords:
Digtal polymerase chain reaction;
Microfluidic chip;
Disease diagnosis;
Single molecule detection;
Review
- From:
Chinese Journal of Analytical Chemistry
2024;52(1):13-21
- CountryChina
- Language:Chinese
-
Abstract:
Digital polymerase chain reaction(dPCR)is a PCR technology that realizes accurate quantification of single-copy nucleic acid molecules by dividing the reaction system into tens of thousands of independent PCR reaction units for single-molecule-level amplification and integrating Poisson distribution.Due to its single-copy sensitivity and accurate quantification without the need of standard curves,dPCR has been widely used in disease diagnosis.By introducing technologies such as stepped emulsification and three-dimensional imaging,dPCR has been greatly improved in terms of accuracy,multiplexability and turnaround time,significantly enhancing its performance in clinical disease diagnosis.Based on this,this paper traced the technological development history of dPCR,gave an overview of its application in detection of tumors,infections and other diseases,and further discussed the challenges and opportunities of the development of dPCR,with the aim of providing a reference for the development and utilization of dPCR in the future,and promoting the high-quality development of molecular technology in clinical testing.
